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Renokine II Testing Service (Research Use Only)

May 8, 2013   //   by renovar   //    //  No Comments

Renokine II Testing Service (Research Use Only)

Renokine II Testing Service (Research Use Only)

Renokine II Testing Service (Research Use Only)

250.00 $
Quantity : 10
Our Renokine II IP-10 Testing Service allows for a researcher to submit samples for testing in our lab.  Results will be delivered via fax or email to the researcher.  Researchers can provide up to 10 samples for testing per order.

 

Purpose The Human IP-10 ELISA is to be used for the quantitative determination in urine.
Principle of the Method The Human IP-10 kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IP-10 is has been coated onto the wells of the microtiter strips provided. Samples of Urine supernatant are pipetted into these wells followed by the addition of a second biotinylated monoclonal antibody.During the first incubation, the IP-10 antigen binds to the immobilized (capture) antibody on one site and to the solution phase biotinylated antibody on a second site.After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of IP-10 present in the original specimen.
Background Information Human IP-10, the interferon-gamma inducible protein-10, also known as CXCL10, is a member of CXC chemokine family (1,2). Human IP-10 is produced mainly by monocytes but also T-cells, fibroblasts and endothelial cells (2). Unlike other CXC chemokines, IP-10 is a chemoattractant for activated lymphocytes, but not resting lymphocytes or neutrophils (2).Human IP-10 is a potent inhibitor of angiogenesis; inhibits neovascularization and exerts antitumor effects (3-6). Human IP-10 inhibits proliferation of human endothelial cells (7) and bone marrow derived hematopoietic progenitors (8).The gene for human IP-10 has been mapped to chromosome 4q2 (9). CXC chemokine receptor (CXCR3) has been identified as the receptor for IP-10 (10)
Ripple Background Information Human IP-10 when identified above a certain concentration in urine has experimentally been demonstrated to indicate acute inflammation, tubulitis, and compromised kidney function.
  General Specifications          
Marker IP-10
Species Human
Platform Microplate Reader
Gene Symbol CXCL10
Gene Aliases C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1
Label Dye Horseradis Peroxidase
Sample Volume 50 µl 
Protein Family Chemokines & Receptors
Incubation Time 4 hours
Detection Method Colorimetric
Research Category Immunology
Sample Urine
Shipping Frozen or On-Ice
Regulatory Statement Research Use Only
  Key Publications (General)
  1. Luster, A.D., et al. (1987) J. Exp. Med. 166:1084-1097.
  2. Farber, J.M. (1997) J. Leukoc. Biol. 61:246-257.
  3. Sgadari, C., et al. (1997) Blood 89:2635.
  4. Arenberg, G., et al. (1996) J. Exp. Med. 184:981.
  5. Angiolillo, A., et al. (1995) Leuk. Lymphoma 19:267.
  6. Luster, A.D., et al. (1993) J. Exp. Med. 178:1057.
  7. Luster, A.D., et al. (1995) J. Exp. Med. 182:219-231.
  8. Sarris, A.H., et al. (1993) J. Exp. Med. 178:1127-1132.
  9. Luster, A.D., et al. (1987) Proc. Natl. Acad. Sci. USA 84:2868-2871.
  10. Loetscher, M., et al. (1996) J. Exp. Med. 184:963-969.
Key Publications (Kidney Related)
  1. Hirt-Minkowski, P., et al. (2012), Detection of Clinical and Subclinical Tubulo-Interstitial Inflammation by the Urinary CXCL10 Chemokine in a Real-Life Setting. American Journal of Transplantation. doi: 10.1111/j.1600-6143.2012.03999.x
  1. Lo D.J., et al. “Chemokines and their receptors in human renal allotransplantation.” Transplantation. 2011 Jan 15;91(1):70-7.
  1. Hu HZ, et.al. “Elevation of CXCR3-binding chemokines in urine indicates acute renal-allograft dysfunction.” American Journal of Transplantation 2004 4:432-7.
  1. Kanmaz T, et al. “Surveillance of acute rejection in baboon renal transplantation by elevation of interferon-gamma inducible protein-10 and monokine induced by interferon-gamma in urine. Transplantation 2004 78:1002-7.
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